In vitro Evaluation of Antioxidant Properties of Different Solvent Extracts of Rumex acetosella Leaves

Hira Baig*, Dildar Ahmed, Saman Zara, Muhammad Ikram Aujla and Muhammad Nadeem Asghar

Department of Chemistry, Forman Christian College University, Ferozpur Road, Lahore (Pakistan).

Corresponding Author E-mail: baighira@gmail.com

ABSTRACT:

A wide range of antioxidant studies were conducted on fractions in various solvents of the leaves of Rumex acetosella including estimation of total phenolics and flavonoids, DPPH free radical scavenging, FRAP, ABTS, phosphomolybdate, reducing power and lipid peroxidation assays. The butanolic fraction showed highest phenolic and flavonoid contents; 203.30 µg/mL of gallic acid equivalent and 745 µg/mL of rutin equivalent respectively. In linoleic acid emulsion and reducing power assays, fractions in all solvents showed strong antioxidant potential. All the fractions efficiently scavenged the DPPH free radical and butanolic fraction had the lowest EC50 (74.95 µg/mL) and TEC50 (2 min). The phosphomolybdate antioxidant activity of the plant extracts ranged from 107.84 - 42.24 µg/mL of AAE (Ascorbic Acid Equivalent). The butanolic fraction had the highest FRAP value (104.23 µg/mL of AAE) and the highest TEAC value (1588.203 mM) in ABTS assay. The chloroform fraction showed the lowest TEAC value (405.359 mM). The polar fractions, having higher phenolics and flavonoids, showed remarkable antioxidant potential, the butanolic fraction being the most potent.

KEYWORDS:

Rumex acetosella; Free radical Scavenging; Antioxidant

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Copy the following to cite this article: Baig H, Ahmed D, Zara S, Aujla M. I, Asghar M. N. In vitro Evaluation of Antioxidant Properties of Different Solvent Extracts of Rumex acetosella Leaves. Orient J Chem 2011;27(4).

Copy the following to cite this URL: Baig H, Ahmed D, Zara S, Aujla M. I, Asghar M. N. In vitro Evaluation of Antioxidant Properties of Different Solvent Extracts of Rumex acetosella Leaves. Orient J Chem 2011;27(4). Available from: http://www.orientjchem.org/?p=24182