Comparison of Conventional HPLC with UPLC Method for Determination of Albuterol Sulfate and it’s Impurities in Pharmaceutical Formulation

P. N. Dalvi, Z. Filmwala, K. Avinash and T Bane*

Analytical Development Laboratory, Cipla Ltd. L. B. S. Marg. Vikhroli, Mumbai - 400 083 India.

ABSTRACT:

A simple, rapid, gradient reversed phase Ultra Performance Liquid Chromatographic method with ultraviolet detection (UPLC-UV) was developed for analysis of Albuterol sulfate and its impurities in Albuterol sulfate inhalation solution pharmaceutical formulation. Chromatography was carried out on a Waters Acquity BEH C18 (1.7μm, 100mm.x 2.1mm) column using 0.02 M Potassium Dihydrogen Orthophosphate as mobile phase A and Acetonitrile as mobile phase B. at a flow rate of 0.150mL/min and a 210 nm detection. Results from table 3 and 4 showed that Ultra Performance Liquid Chromatography exhibited a rapid and separation efficiency superior to that of existing conventional HPLC method.

KEYWORDS:

UPLC; HPLC; Albuterol Sulfate; Time; Cost

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Copy the following to cite this article: Dalvi P. N, Filmwala Z, Avinash K, Bane T. Comparison of Conventional HPLC with UPLC Method for Determination of Albuterol Sulfate and it’s Impurities in Pharmaceutical Formulation. Orient J Chem 2008;24(2).

Copy the following to cite this URL: Dalvi P. N, Filmwala Z, Avinash K, Bane T. Comparison of Conventional HPLC with UPLC Method for Determination of Albuterol Sulfate and it’s Impurities in Pharmaceutical Formulation. Orient J Chem 2008;24(2).