RP-HPLC Method Development for Estimation of Atropine Sulphate in Bulk Drug

A. S. Raghuwanshi and U. K. Jain

Bhopal Institute of Technology an Science-Pharmacy, Bhopal (India).

ABSTRACT:

An isocratic RP-HPLC method was developed for the estimation of atropine sulphate in bulk. The separation was achieved with a C18 column using mobile phase comprising of methanol:5 mmol potassium dihydrogen phosphate buffer (50: 50, v/v), the pH of which was kept unadjusted. The flow rate was kept at 1 mL min-1 and analysts was screened with UV detector at 264 nm. The retention time for atropine sulphate was found to be 4.797 minutes. The LOD and LOQ for atropine sulphate were found to be 23.07 and 69.91 ng mL-1, respectively. The described method was precise and sensitive.

KEYWORDS:

Atropine Sulphate; Reverse Phase HPLC

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Copy the following to cite this article: Raghuwanshi A. S, Jain U. K. RP-HPLC Method Development for Estimation of Atropine Sulphate in Bulk Drug. Orient J Chem 2009;25(3).

Copy the following to cite this URL: Raghuwanshi A. S, Jain U. K. RP-HPLC Method Development for Estimation of Atropine Sulphate in Bulk Drug. Orient J Chem 2009;25(3). Available from: http://www.orientjchem.org/?p=23078