Noval estimation of cefadroxil in tablet dosage forms by RP-HPLC

Roopam Devaliya and U.K. Jain*

Bhopal Institute of Technology and Science-Pharmacy, Bhojpur Road, Bhopal - 462 045 (India).

ABSTRACT:

A simple specific, accurate and precise reverse phase high performance liquid chromatographic method was developed and validated for the estimation of Cefadroxil in tablet dosage forms. A Hypersil ODS column (4.6 x 250mm, 5μm) in isocratic mode with mobile phase phosphate buffer pH 5.0 and acetonitrile ratio (96:4) was used. The flow rate was 1.0 ml/min. The detection was carried out at 254 nm and retention time of Cefadroxil was found to be 8.1 min. The method was validated for specificity, linearity, accuracy, precision, and limit of quantification, limit of detection & robustness. The limit of detection and limit of quantification for estimation of Cefadroxil were found to be 0.4 and 1.3 mg, respectively. The recoveries of Cefadroxil in tablet dosage were found to be in the range of 99.03-101.8%. Proposed method was successfully applied for the quantitative determination of Cefadroxil in tablet dosage forms.

KEYWORDS:

Cefadroxil; b lactum antibiotics; RP-HPLC; validation

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Copy the following to cite this article: Devaliya R, Jain U. K. Noval estimation of cefadroxil in tablet dosage forms by RP-HPLC. Orient J Chem 2009;25(4).

Copy the following to cite this URL: Devaliya R, Jain U. K. Noval estimation of cefadroxil in tablet dosage forms by RP-HPLC. Orient J Chem 2009;25(4). Available from: http://www.orientjchem.org/?p=22793